culture conditions minimal glucose medium (Teknova)
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culture conditions minimal glucose medium
Culture Conditions Minimal Glucose Medium, supplied by Teknova, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mops+minimal+media/MOPS+Minimal+Media+Kit/pm42024128-220-3-14
Average 95 stars, based on 54 article reviews
Culture Conditions Minimal Glucose Medium, supplied by Teknova, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mops+minimal+media/MOPS+Minimal+Media+Kit/pm42024128-220-3-14
Average 95 stars, based on 54 article reviews
culture conditions minimal glucose medium - by Bioz Stars,
2026-09
95/100 stars
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other:Article Title: Metabolic interplay drives population cycles in a cross-feeding microbial community Article Snippet: Precultures of Δ tyrA and Δ pheA were used to inoculate 1 mL of fresh Article Title: Metabolic interplay drives population cycles in a cross-feeding microbial community. Article Snippet: Precultures of ΔtyrA and ΔpheA were diluted into 1mL of fresh Article Title: Metabolic interplay drives population cycles in a cross-feeding microbial community Article Snippet: Cultures were then centrifuged for 5 min at 3000 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times g$$\end{document} × g , and the cell pellet was washed once with Article Title: Metabolic interplay drives population cycles in a cross-feeding microbial community. Article Snippet: Precultures of ΔtyrA and ΔpheA were used to inoculate 1mL of fresh Article Title: Metabolic interplay drives population cycles in a cross-feeding microbial community Article Snippet: Precultures of Δ tyrA and Δ pheA were diluted as monocultures ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$n=3$$\end{document} n = 3 ) into 4 mL of fresh Article Title: Metabolic interplay drives population cycles in a cross-feeding microbial community. Article Snippet: Cultures were then centrifuged for 5min at 3000 × g, and the cell pellet was washed once with Bacteria:Article Title: Transcriptome data set of Cellvibrio japonicus grown using β-glucan media Article Snippet: .. C. japonicus Ueda107 was obtained from the National Collections of Industrial and Marine Bacteria (Aberdeen, UK) as a lyophilized powder and revived using Article Title: Transcriptome data set of Cellvibrio japonicus grown using β-glucan media. Article Snippet: .. C. japonicus Ueda107 was obtained from the National Collections of Industrial and Marine Bacteria (Aberdeen, UK) as a lyophilized powder and revived using |
![Schematic depicting the genetic modifications to enable the overaccumulation of NADPH (Δ pgi , Δ edd , Δ qor , Δ sthA ) and the deletion of aceA to prohibit growth on acetate as a carbon source. Pathways enabling growth on a mixture of acetate and glucose are shown below and highlighted (red: acetaldehyde (strain APEQS_PduP), yellow: 3-HB (strain APEQS_3-HB), blue: mevalonate (strain APEQS_MEV_sa)). b, Simplified metabolic stoichiometries showing only bioavailable carbon and relevant reducing equivalents and assuming all carbon flows to acetyl-CoA (our pathways’ precursor molecule). Simplified stoichiometry of E. coli fermentative metabolism and APEQS metabolism when grown on glucose, showing redox-balanced fermentative or rescue pathways below. Ethanol fermentation requires less acetyl-CoA (green) than is produced from one glucose when redox balanced, reflecting its suitability as a fermentation pathway. Partially reducing pathways consume more acetyl-CoA (red) than is made available per unit glucose when redox balanced, indicating their inability to resolve redox balance in APEQS without acetate co-feeding. c, Unsuccessful growth coupling of strain APEQS_PduP in the absence of acetate (n=3). d, Unsuccessful growth coupling of strain APEQS_3-HB in the absence of acetate (n=3). e, Unsuccessful growth coupling of strain APEQS_MEV_sa in the absence of acetate (n=3). f, Successful growth coupling of strain APEQS_PduP when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). g, Successful growth coupling of strain APEQS_3-HB when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). h, Successful growth coupling of strain APEQS_MEV_sa when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). All experiments were conducted in a <t>MOPS</t> <t>medium</t> containing 2% glucose with or without 100 mM acetate. Various concentrations of IPTG were added to modulate the induction of the three partially reducing pathways (high [IPTG]: blue (0.5 mM for p15A-based A5c backbone; 0.05 mM for ColE1-based pQE backbone), medium [IPTG]: purple (0.05 mM for p15A-based A5c backbone; 0.005 mM for ColE1-based pQE backbone), no IPTG: red). An empty vector control was included to demonstrate growth without leaky expression (orange). All growth experiments were repeated a minimum of 3 times and showed identical results.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_23/10__64898_slash_2026__04__01__713023/10__64898_slash_2026__04__01__713023___F1.large.jpg)
